Analysis Note

ControlPOSITIVE CONTROL:

For western blot, load 5-10 µg of whole cell lysate prepared from the human epidermoid carcinoma cell line, A431.

Application

Anti-Paxillin Antibody, clone 349 detects level of Paxillin & has been published & validated for use in ELISA, WB, IC, IH.

Research CategorySignaling

Western blot: 1:1,000-1:10,000

ELISA

Immunohistochemistry 1:900 in Placenta

Immunocytochemistry

Immunoprecipitation

Optimal working dilutions must be determined by end user.

Research Sub CategoryCytoskeletal Signaling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Affinity purified phosphotyrosine-containing proteins from Rous sarcoma virus-transformed chick embryo fibroblasts.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-581

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified immunoglobulin. Liquid in aqueous buffered solution containing 0.09% sodium azide.

Format: Purified

Specificity

Reacts strongly with two bands on Western blots of total cell lysate from chick embryo fibroblasts or rat brain. The lower molecular band is believed to be either an isoform or a degradation product of the 68 kD protein, since it is found also on SDS-PAGE of affinity-purified paxillin.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.